KMID : 0620920010330040269
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Experimental & Molecular Medicine 2001 Volume.33 No. 4 p.269 ~ p.275
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Transcriptional repression of Type¥° procollagen genes during adipocyte differentiation
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In San Kim/Tac Ghee Yi
Hee Man Choi/Rang Woon Park/Kun Young Sohn/In San Kim
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Abstract
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Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both ¥á1 and ¥á2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.
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KEYWORD
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Procollagen, adipocyte differentiation, transcription, repression,
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